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Determination of Lactose by an Enzymatic Method

✍ Scribed by Kleyn, Dick H.


Book ID
123110109
Publisher
American Dairy Science Association
Year
1985
Tongue
English
Weight
631 KB
Volume
68
Category
Article
ISSN
0022-0302

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A fluoroenzymatic microdetermination of lactose in tissue culture medium is described. The method is based on the conversion of lactose to galactonic acid by the enzymes galactosidase and galactose dehydrogenase. The resultant reduction of NAD to NADH is measured fluorometrically. Before assaying fo