✦ LIBER ✦

Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry

✍ Scribed by John M. Miles; W.Frederick Schwenk; Kelly L. McClean; Morey W. Haymond

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 463 KB
Volume: 141
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90432-9

No coin nor oath required. For personal study only.

✦ Synopsis

A simple and reliable method for the determination of ketone body turnover in vivo using a primed, continuous infusion of [3,4-'"C2]acetoacetate is described. Mole percent enrichment of @-['3C2]hydroxybutyrate and [lrC,Jacetoacetate is determined by gas chromatography/mass spectrometry using electron-impact ionization and selected ion monitoring. Ketone body flux data are provided from preliminary dog experiments. The method is readily applicable to the study of ketone body metabolism in both laboratory animals and humans.

KEY WORDS: isotope dilution methods; gas chromatography/mass spuctrometry; stable isotopes; ketone bodies.

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