Stable isotope labelling in biosynthetic studies of dill ether, using enantioselective multidimensional gas chromatography, online coupled with isotope ratio mass spectrometry

✍ Scribed by Sylvia Reichert; Dirk Fischer; Sven Asche; Armin Mosandl

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 132 KB
• Volume: 15
• Category: Article
• ISSN: 0882-5734
• DOI: 10.1002/1099-1026(200009/10)15:5<303::aid-ffj912>3.0.co;2-2

✦ Synopsis

R:S#!Limonene and "¦:-#!p!menth!0!en!8!ol as glucoside were synthesized as 02 C!labelled stereoisomers[ Leaves of Anethum raveolens L[ were fed with aqueous solutions of the labelled precursors[ Feeding experiments and subsequent analysis of 02 C: 01 C ratios were carried out by means of enantioselective multidimensional gas chromatography\ online coupled with isotope ratio mass spectrometry[ The dill plants were able to convert the fed menthenol!isomers into the corresponding dill ether stereoisomers[ Nevertheless\ it was proved that the stereospeci\_c hydration of "R#!limonene leads to "3R\ 7S#!p!menth!0!en!8!ol as the \_rst step in dill ether biosynthesis[ Based on these results\ the biogenesis of dill ether in A[ raveolens L[ is discussed[