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Determination of inositol 1,4,5-trisphosphate levels in Dictyostelium by isotope dilution assay

โœ Scribed by Peter J.M. Van Haastert


Book ID
107714670
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
565 KB
Volume
177
Category
Article
ISSN
0003-2697

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Modification of a Competitive Protein Bi
โœ P. Gerwins ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 383 KB

A modification of a competitive protein-binding method for the determination of inositol \(1,4,5\)-trisphosphate ( \(\left.\operatorname{Ins}(1,4,5) P_{3}\right)\), facilitated by the use of a cell harvester, is described. The method is based on the competition of \(\left[{ }^{3} H\right] I n s(1,4,