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A competition binding assay for determination of the inositol (1,4,5)-trisphosphate content of human leucocytes

✍ Scribed by Peter H. Nibbering; Timo P.L. Zomerdijk; Peter J.M. van Haastert; Ralph van Furth


Book ID
115763718
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
540 KB
Volume
170
Category
Article
ISSN
0006-291X

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Modification of a Competitive Protein Bi
✍ P. Gerwins πŸ“‚ Article πŸ“… 1993 πŸ› Elsevier Science 🌐 English βš– 383 KB

A modification of a competitive protein-binding method for the determination of inositol \(1,4,5\)-trisphosphate ( \(\left.\operatorname{Ins}(1,4,5) P_{3}\right)\), facilitated by the use of a cell harvester, is described. The method is based on the competition of \(\left[{ }^{3} H\right] I n s(1,4,