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A new competition binding assay for determination of the cAMP content of human leukocytes

✍ Scribed by Timo P.L. Zomerdijk; Peter H. Nibbering; Anja C. Bezemer; Clemens W.G.M. Löwik; Ralph van Furth

Book ID
115764113
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
385 KB
Volume
178
Category
Article
ISSN
0006-291X

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✍ P. Gerwins 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 383 KB

A modification of a competitive protein-binding method for the determination of inositol \(1,4,5\)-trisphosphate ( \(\left.\operatorname{Ins}(1,4,5) P_{3}\right)\), facilitated by the use of a cell harvester, is described. The method is based on the competition of \(\left[{ }^{3} H\right] I n s(1,4,