Determination of iga antibodies to human cytomegalovirus by enzyme-linked immunosorbent assay (elisa)

✍ Scribed by Esther Levy; Israel Sarov

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 524 KB
• Volume: 6
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890060308

✦ Synopsis

Abstract

A sensitive enzyme‐linked immunosorbent assay (ELISA) is described for detection of IgA antibodies to human cytomegalovirus (CMV). The antigen consisted of a sonicated extract of CMV infected human embryo cells. The tested sera were absorbed with staphylococcus aureus (strain Cowan 1) before analysis. Rabbit antihuman IgA peroxidase conjugate was used to detect human IgA bound to viral antigen. In parallel, IgM and IgG antibodies to CMV were studied by ELISA and by the immunoperoxidase antibody to membrane antigen (IPAMA) technique, respectively. CMV IgA was detected in high titers by ELISA in eight out of nine CMV patients. The maximal IgA titers were generally lower than the IgM titers detected by ELISA. No CMV IgA antibodies (titer < 20) were detected by ELISA in 57 control sera (healthy adults, hospitalized patients with various other diseases), paired sera of five patients with acute herpes simplex infection, two patients with Epstein‐Barr infections, one patient with varicella, and one with zoster infections. The potential application of the ELISA CMV IgA technique in serodiagnosis of CMV infections is discussed.