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Detection of human cytomegalovirus-specific iga antibodies in colostrum by enzyme-linked lmmunosorbent assay (elisa)

✍ Scribed by Avraham Pinku; Hava Haikin; Maureen G. Friedman; Dr. Israel Sarov

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
356 KB
Volume
9
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

Fifty women were examined after delivery for the prevalence of antibodies to human cytomegalovirus in colostrum and sera. Eighty percent of them had specific CMV IgG antibodies in the sera, as determined by the immunoperoxidase antibody to membrane antigen (IPAMA) technique. Of the CMV‐seropositive women, 60% had specific CMV IgA antibodies in high titer in the colostrum as determined by enzyme‐linked immunosorbent assay (ELISA). In only two of the seropositive women were specific CMV IgA antibodies detected in the sera as well. The significance of specific CMV IgA antibodies in colostrum as protection against perinatal infection and the mechanism of this production will be discussed.

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✍ Gillian E. Morris; R. Marie Coleman; Jennifer M. Best; Bonnie B. Benetato; Andé 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 457 KB

Enzyme-linked immunosorbent assays were used to detect IgG and IgA antibodies to herpes simplex virus (HSV), varicella-zoster virus (VZV), cytomegalovirus (CMV), and rubella virus in sera from 68 adult female gynaecological patients. Of the patients who had virus-specific IgG antibodies, the proport