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Development of a competitive enzyme-linked immunosorbent assay for detecting cytomegalovirus antibody

✍ Scribed by T. G. Wreghitt; Joanna Hicks; J. J. Gray; Candice O'Connor

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 622 KB
Volume: 18
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890180204

No coin nor oath required. For personal study only.

✦ Synopsis

A competitive enzyme-linked immunosorbent assay (ELISA) for detecting cytomegalovirus (CMV) antibody was developed. The competitive ELISA was five times more sensitive than the complement fixation test (CFT) and twice as sensitive as indirect ELISA. Testing of paired sera from cardiac transplant patients taken before and after transplantation showed good correlation between results of competitive and indirect ELISA and CFT. The competitive ELISA was more successful than CFT or indirect ELISA in detecting passively acquired antibody, but detection of CMV antibody by competitive ELISA immediately after primary CMV infection was unreliable, possibly because of the high affinity of the monoclonal antibody chosen for the horseradish peroxidase conjugate. However, competitive ELISA may well prove to be more suitable than indirect ELISA for detecting CMV antibody in blood donations.

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