Determination of glucocorticoid receptors in human leukemias
β Scribed by Ho, A. D. ;Hunstein, W. ;Schmid, W.
- Publisher
- Springer-Verlag
- Year
- 1980
- Tongue
- English
- Weight
- 364 KB
- Volume
- 58
- Category
- Article
- ISSN
- 1432-1440
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π SIMILAR VOLUMES
Glucocorticoids have been used widely in the treatment of leukemia; however, response has been variable. Glucocorticoid receptors have been implicated as the molecular basis of response to this hormonal therapy. Glucocorticoid receptor levels have been found to extend over a wide range in normal hum
## Abstract Glucocorticoid receptors were measured from leukemic cells of five patients with hairyβcell leukemia as well as a cell line (JOKβ1) derived from one of the patients studied and grown in continuous culture __in vitro__. Receptor analyses were accomplished using a wholeβcell binding assay
## Abstract Membraneβbound immunoglobulins, receptors for the Fc fragment of IgG and receptors for the third component of human or murine complement were used as B cell membrane markers to study eripheral blood lymphocytes from twentytwo patients with chronic lymphatic leukemia (CLL), five patients
## Abstract We have established optimal conditions for the measurement of glucocorticoid receptors (GR) in human white cells using a wholeβcell binding assay with [^3^H]dexamethasone as the ligand, and the subsequent determination of the GR content in normal human lymphocytes and in leukemic cells