The utility of the fluorescence derivatixation reagents, 4-(N,N-dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-l-yl)\_2,1,3-benzoxadiaxole [ R( + J-DBD-Pro-CCC1 and S( -)-DBD-Pro-CCC11 with compounds having various functional groups has been evaluated. Enantiomers of DBD-Pro-COCI were equally re
Determination of free fatty acids in blood, tagged with 4-(2-carbazoylpyrrolidin-1-yl)-7-(N, N-dimethylaminosulfonyl)-2, 1, 3-benzoxadiazole, by high-performance liquid chromatography with fluorescence detection
β Scribed by Toshimasa Toyo'oka; Maki Takahashi; Asako Suzuki; Yasuko Ishii
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 597 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0269-3879
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A highly sensitive method, based on fluorescence derivatization with 4-N,N-dimethylaminosulfonyl-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ), was developed for the determination of D-and L-lactic acid. High-performance liquid chromatographic separation of the lactic acid derivative was achieved using
D-Amino acids in food and biological samples labeled with R(-)- and S(+)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N, N-dimethylaminosulfonyl)-2,1,3-benzoxadiazoles (DBD-PyNCS) were separated by reversed-phase chromatography and detected fluorometrically at 550 nm (excitation at 460 nm). DL-Amino acids
## Abstract The usefulness of __R__(β)β4β(3βisothiocyanatopyrrolidinβ1βyl)β7β(__N,N__βdimethylaminosulfonyl)β2,1,3βbenzoxadiazole [__R__(β)βDBDβPyNCS], a fluorescent chiral tagging reagent, for the determination of racemic amines and amino acids, was studied. The reagent reacted with Ξ²βblockers sel