Fluorimetric determination of D- and L-lactate derivatized with 4-(N,N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) by high-performance liquid chromatography
✍ Scribed by Takeshi Fukushima; Sinya Adachi; Hideaki Ichihara; Salma Al-Kindy; Kazuhiro Imai
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 86 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0269-3879
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✦ Synopsis
A highly sensitive method, based on fluorescence derivatization with 4-N,N-dimethylaminosulfonyl-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ), was developed for the determination of D-and L-lactic acid. High-performance liquid chromatographic separation of the lactic acid derivative was achieved using an octadecylsilica column followed by enantiomeric separation on a phenylcarbamoylated b-cyclodextrin chiral column. The separation factor for D,L-lactic acid derivatives was 1.30 using MeOH/H 2 O (80/20) as a mobile phase, and the detection limits were approximately 360 and 300 fmol on column for D-and L-lactic acid derivative, respectively. The proposed method was applied to determine D-and L-lactate in a wine and a lactic drink. Both D-and L-lactate could be determined simultaneously, with the precisions ranging from 3.50 to 5.78% for intra-day and 4.28-9.92% for inter-day determinations. The relative recovery was in the range of 91.6-100%.