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Determination of fat peroxides in the presence of phospholipids

✍ Scribed by L. Hartman

Publisher
John Wiley and Sons
Year
1954
Tongue
English
Weight
526 KB
Volume
5
Category
Article
ISSN
0022-5142

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✦ Synopsis

Abstract

A colorimetric method of estimating fat peroxides in materials such as animal tissues has been developed, which is free from interference by phospholipids. The peroxides are reduced with ferrous chloride in benzene‐methanol solution and the amount of ferric iron produced is determined by the addition of 2 : 6‐dichlorophenolindophenol and pyro‐phosphoric acid. The extent of phospholipid interference with the ferric thiocyanate method of peroxide estimation has been measured by comparison with the new method. This interference has been found on the whole appreciable owing to the solubility of phospholipids in solvents used for fat extraction.

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