Determination of Epinephrine by Flow Injection Analysis Coupled Ag(III) Complex-Luminol Chemiluminescence Detection

✍ Scribed by Jiangbo BAI; Hongmei SHI; Yingze ZHANG; Dehu TIAN; Xiangdong XU; Weijun KANG

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 146 KB
• Volume: 27
• Category: Article
• ISSN: 0256-7660
• DOI: 10.1002/cjoc.200990123

✦ Synopsis

Abstract

A new Ag(III) complex‐luminol chemiluminescent system which was applied to the determination of epinephrine is firstly reported. Based on the enhancing effect of epinephrine on the chemiluminescence reaction of luminol with [Ag(HIO~6~)~2~]^5−^ in alkaline solution, a highly sensitive chemiluminescence (CL) detection by flow injection analysis (FIA) was developed for epinephrine. Under the optimum conditions, CL intensity was proportional to concentration of epinephrine in the 1.0×10^−9^–1.0×10^−7^ mol·L^−1^ range. The limit of detection was 8.0×10^−10^ mol·L^−1^ for epinephrine (3__δ__), with a relative standard deviation (n=11) of 2.9% for 1.5×10^−8^ mol·L^−1^ epinephrine. The method validation was done with epinephrine determinations in commercial pharmaceutical products. The mechanism of the reactions was also discussed.