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Determination of double bond position in tri- to hexaenoic fatty acids by mass spectrometry

✍ Scribed by Brigitte Schmitz; Heinz Egge

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
503 KB
Volume
25
Category
Article
ISSN
0009-3084

No coin nor oath required. For personal study only.

✦ Synopsis

Methylesters of polyenoic fatty acids (FAE) with up to six double bonds can be transformed to polyhydmxy derivatives by treatment with OsO 4. Under low energy electron impact the trimethylsilyloxy (O-TMS) derivatives of these polyenoic fatty acids exhibit mass spectro metric fragmentation patterns that are straightforward due to the almost exclusive cleavage of the C-C bonds in between vicinal O-TMS groups. Thus it is possible to localise original double bond positions and also to predict fragmentation patterns of hitherto unknown fatW acid species. The method is applicable to polyenoic compounds with isolated as well as conjugated double bonds.

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