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Determination of fatty acid positions in native lipid A by positive and negative electrospray ionization mass spectrometry

โœ Scribed by Stefano Sforza; Alba Silipo; Antonio Molinaro; Rosangela Marchelli; Michelangelo Parrilli; Rosa Lanzetta

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
293 KB
Volume
39
Category
Article
ISSN
1076-5174

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โœฆ Synopsis

Abstract

Lipid A is the endotoxic principle of the lipopolysaccharide fraction from Gramโ€negative bacteria. It is involved in the elicitation of cytokine production that leads to massive inflammation and to septic shock as a lethal consequence. For this reason, the structural elucidation of lipopolysaccharides from toxic Gramโ€negative bacteria is an important and complicated task, mainly owing to its natural heterogeneity. Here, a new methodology to infer the distribution of the primary and secondary acyl residues is described, based on electrospray ionization mass spectrometry (ESIโ€MS) of intact lipid A under high cone voltage in order to achieve inโ€source fragmentation. Under these conditions, acyl fragmentation is induced and a different regioselective cleavage of secondary fatty acids is observed in positive and negative ESIโ€MS, allowing the rapid identification of the lipid A structure. Copyright ยฉ 2004 John Wiley & Sons, Ltd.

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