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Determination of disulfide bonds in highly bridged α-dendrotoxin by matrix-assisted laser desorption/ionization mass spectrometry

✍ Scribed by Hélène Belva; Chrystel Valois; Catherine Lange


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
80 KB
Volume
14
Category
Article
ISSN
0951-4198

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✦ Synopsis


A methodology which combines tryptic digestions, 1,4-dithiothreitol reduction, and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) allows verification of the location of the disulfide bridges in alpha-dendrotoxin (C7-C57, C16-C40 and C32-C53). Copyright 2000 John Wiley & Sons, Ltd.


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