✦ LIBER ✦

Determination of derivatized clenbuterol by use of gas chromatography and tandem mass spectrometry on an ion trap using negative ion chemical ionization

✍ Scribed by JeongAe Lee; Dong-seok Lho; Myungsoo Kim; Won Lee; Yunje Kim

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 151 KB
Volume: 12
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19981015)12:19<1366::aid-rcm324>3.0.co;2-i

No coin nor oath required. For personal study only.

✦ Synopsis

Doping control studies for the detection of clenbuterol in human urine was studied. Confirmation was achieved by trifluoroacetylation after cyclic fluorophenylboronation to produce FPBA-clenbuterol, for derivatization over 10 min at 80 °C, measured using negative chemical ionization (NICl) and NICl-tanden mass spectrometry (MS/MS) on an ion trap mass spectrometer. In the NICl-MS/MS mode, the m/z 440 ion was selected as the precursor ion. This ion may be formed from FPBA-clenbuterol trifluoroacetate, which has a molecular weight of 476, having lost HCl. Derivatized clenbuterol was detected to 50 fg/mL, in spiked human urine. We applied the above procedure to two positive samples, one as a proficiency test and the other for reaccreditation, and achieved good results.

📜 SIMILAR VOLUMES

Determination of anabolic steroids by ga

Determination of anabolic steroids by gas chromatography/negative-ion chemical ionization mass spectrometry and gas chromatography/negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization

✍ Man Ho Choi; Bong Chul Chung; Won Lee; Un Chul Lee; Yunje Kim 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 118 KB 👁 2 views

A gas chromatography/mass spectrometry (GC/MS) method is described which uses negative ion chemical ionization (NCI) and tandem mass spectrometry (MS/MS) for the determination of eight anabolic steroids in human urine. Eight anabolic steroids were derivatized by heptafluorobutyric anhydride (HFBA),

Determination of four anabolic steroid m

Determination of four anabolic steroid metabolites by gas chromatography/mass spectrometry with negative ion chemical ionization and tandem mass spectrometry

✍ Man Ho Choi; Bong Chul Chung; Myungsoo Kim; Jeongeun Choi; Yunje Kim 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB 👁 2 views

A gas chromatography/mass spectrometry method is described which uses negative ion chemical ionization and tandem mass spectrometry for the determination of anabolic steroid metabolites. Four anabolic steroid metabolites to be derivatized by Pentafluoropropionic anhydride (PFPA) were determined usin

Analysis of alkenes by copper ion chemic

Analysis of alkenes by copper ion chemical ionization gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry

✍ Fordham, P. J.; Chamot-Rooke, J.; Giudice, E.; Tortajada, J.; Morizur, J.-P. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB 👁 2 views

A novel chemical ionization/fast atom bombardment (CI/FAB) source was used to analyse alkenes by chemical ionization mass spectrometry (CI-MS) using copper ions as the ionizing agent. The Cu Y -CI mass spectra showed abundant pseudomolecular adduct ions [alkene-Cu] Y and characteristic fragment ions

Determination of the sensitivity of an e

Determination of the sensitivity of an external source ion trap tandem mass spectrometer using dimethyl ether chemical ionization

✍ Wu, Hui-Fen; Lin, Ya-Ping 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 50 KB 👁 1 views

## Determination of the Sensitivity of an External Source Ion Trap Tandem Mass Spectrometer Using Dimethyl Ether Chemical Ionization Tandem mass spectrometry is a rapid and powerful analytical technique that can provide trace mixture analysis and structure elucidation. The ion trap mass spectromet

Analysis of a commercial preparation of

Analysis of a commercial preparation of erythromycin estolates by tandem mass spectrometry and high performance liquid chromatography/electrospray ionization tandem mass spectrometry using an ion trap mass spectrometer

✍ Chien-Chen Lai; Pei-Lun Tsai; Cheng Yu; Guor-Rong Her 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 114 KB 👁 2 views

Because of the lack of a UV chromophore and their much smaller abundances in comparison with the major component, the minor components in erythromycin estolate preparations are difficult to analyze by high performance liquid chromatography ultraviolet (HPLC-UV). Tentative assignment of the major and

Preparation of Deuterated Diclofenac for

Preparation of Deuterated Diclofenac for Use as an Internal Standard in Quantitative Measurement by Gas Chromatography Negative-ion Chemical Ionization Mass Spectrometry

✍ H. J. Leis; G. Fauler; H. Gleispach; W. Windischhofer 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 217 KB 👁 1 views