✦ LIBER ✦

Determination of four anabolic steroid metabolites by gas chromatography/mass spectrometry with negative ion chemical ionization and tandem mass spectrometry

✍ Scribed by Man Ho Choi; Bong Chul Chung; Myungsoo Kim; Jeongeun Choi; Yunje Kim

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 164 KB
Volume: 12
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19981130)12:22<1749::aid-rcm395>3.0.co;2-s

No coin nor oath required. For personal study only.

✦ Synopsis

A gas chromatography/mass spectrometry method is described which uses negative ion chemical ionization and tandem mass spectrometry for the determination of anabolic steroid metabolites. Four anabolic steroid metabolites to be derivatized by Pentafluoropropionic anhydride (PFPA) were determined using gas chromatography/mass spectrometry (GC/MS) with negative chemical ionization (NCI) and NCI/MS/MS. The repeatability and reproducibility of this procedure were in the range of 5.3-9.7% and 6.1-10.2%, respectively. This method of derivatization with PFPA for NCI and NCI/MS/MS was useful to determine four metabolites of nandrolone, dromostanolone, methenolone and boldenone. The derivatized metabolites of boldenone could be detected to 2 ppb and the other three steroids could be detected to 25 ppb in urine at a signal-to-noise ratio of S/N = 3.

📜 SIMILAR VOLUMES

Determination of anabolic steroids by ga

Determination of anabolic steroids by gas chromatography/negative-ion chemical ionization mass spectrometry and gas chromatography/negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization

✍ Man Ho Choi; Bong Chul Chung; Won Lee; Un Chul Lee; Yunje Kim 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 118 KB 👁 1 views

A gas chromatography/mass spectrometry (GC/MS) method is described which uses negative ion chemical ionization (NCI) and tandem mass spectrometry (MS/MS) for the determination of eight anabolic steroids in human urine. Eight anabolic steroids were derivatized by heptafluorobutyric anhydride (HFBA),

Analysis of alkenes by copper ion chemic

Analysis of alkenes by copper ion chemical ionization gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry

✍ Fordham, P. J.; Chamot-Rooke, J.; Giudice, E.; Tortajada, J.; Morizur, J.-P. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB 👁 1 views

A novel chemical ionization/fast atom bombardment (CI/FAB) source was used to analyse alkenes by chemical ionization mass spectrometry (CI-MS) using copper ions as the ionizing agent. The Cu Y -CI mass spectra showed abundant pseudomolecular adduct ions [alkene-Cu] Y and characteristic fragment ions

Determination of derivatized clenbuterol

Determination of derivatized clenbuterol by use of gas chromatography and tandem mass spectrometry on an ion trap using negative ion chemical ionization

✍ JeongAe Lee; Dong-seok Lho; Myungsoo Kim; Won Lee; Yunje Kim 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 151 KB 👁 1 views

Doping control studies for the detection of clenbuterol in human urine was studied. Confirmation was achieved by trifluoroacetylation after cyclic fluorophenylboronation to produce FPBA-clenbuterol, for derivatization over 10 min at 80 °C, measured using negative chemical ionization (NICl) and NICl-

Characterization of stereoisomeric furan

Characterization of stereoisomeric furanosidic derivatives by direct chemical ionization mass spectrometry and liquid secondary ion mass spectrometry with tandem mass spectrometry. (Part II)

✍ C. Borges; M. A. Almoster Ferreira; H. Van den Heuvel; M. Claeys 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 186 KB 👁 2 views

The present study deals with the mass spectrometric characterization of furanosidic compounds containing a 1,2-O-isopropylidene group using different mass spectrometric techniques: chemical ionization mass spectrometry and liquid secondary ion mass spectrometry together with metastable ion analysis

Stable Isotope Labelling and Determinati

Stable Isotope Labelling and Determination of Ketroprofen in Human Plasma and Urine by Gas Chromatography/Negative Ion Chemical Ionization Mass Spectrometry

✍ Leis, H. J.; Leis, M.; Windischhofer, W. 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 482 KB 👁 1 views

A method for the quantitative measurement of the non-steroidal anti-inflammatory drug ketoprofen in human plasma and urine is presented. The assay is based on gas chromatographylnegative ion chemical ionization mass spectrometry. The preparation of stable isotope-labelled ketoprofen for use as an in

Identification of cyclopropene triacylgl

Identification of cyclopropene triacylglycerols by high-temperature gas chromatography and high-temperature gas chromatography–negative chemical ionization mass spectrometry

✍ Reiner Aichholz; Volker Spitzer; Eberhard Lorbeer 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 115 KB 👁 1 views

The seed oil of Choriosa speciosa, which is known to be rich in cyclopropene triacylglycerols, has been investigated by high-temperature gas chromatography using glass capillary columns coated with the stationary phase SOP-50 Ž . 50% diphenyl᎐50% dimethylpolysiloxane, methoxy terminated . Cycloprope