Leucine dehydrogenase/r.-amino acid oxidase was proposed as an enzymatic conversion system for ammonia and its application to amperometric assay of creatinine was investigated. Ammonia formed by creatinine deiminase catalyzed hydrolysis of creatinine was converted to L-leucine by leucine dehydrogena
Determination of creatinine with a sensor based on immobilized glutamate dehydrogenase and creatinine deiminase
โ Scribed by Kunio Kihara; Eika Yasukawa
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 376 KB
- Volume
- 183
- Category
- Article
- ISSN
- 0003-2670
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A method for the automated analysis of creatinine in serum using immobilized enzymes in column form which does not require a blank correction and can be run in a single buffer of pH 7.5 is described. The method was based on the determination of ammonia formed by the action of creatinine deiminase. E
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