Amperometric flow injection determination of fructose in honey with a carbon paste sensor based on fructose dehydrogenase
✍ Scribed by Josefina Parellada; Elena Domínguez; Victor M. Fernández
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 647 KB
- Volume
- 330
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
An enzyme carbon paste electrode for quantitation of fructose in a flow injection analysis (FTA) system is developed. The sensor is based on a commercially available pyrrolo quinoline quinone (PQQ)-fructose dehydrogenase. The enzyme is introduced into the carbon paste matrix and addition of polyetbyleneirnine (PEI) allows an unmediated anodic current to be achieved at 400mV vs. Ag/AgCl after injections of fructose in the PIA system. Operating at 4OOmV, at a flow rate of 1 .O ml rnin-' and with 20 ~1 injection volume, the analytical characteristics of the sensor are: limit of detection 75 @I (S/N 3), linearity up to lOmM, sensitivity 3nArnM-', complete specificity for fructose and operational stability for 10h. The biosensor-FIA system has been validated by liquid chromatography-refractive index (LC-RI) for the detection of fructose in honey. Results prove tbe reliability of the biosensor for the quantitation of fructose in honey samples.