Determination of clenbuterol residues by liquid chromatography-electrospray mass spectrometry

✍ Scribed by Laurent Debrauwer; Georges Bories

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 764 KB
• Volume: 275
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80297-x

✦ Synopsis

The apphcatlon of electrospray lonlzatlon to the mass spectrometnc determmatlon of clenbuterol was mvestrgated Usmg electrospray, the energy of the eons extracted mto the vacuum system can be controlled and, therefore, colhs~onally actuated chssoclations (with residual gas) can be Induced m the h&-pressure region of the electrospray lonlzatlon source Accordmg to the energy thus conferred to the eons, the fragmentation of the molecule can be readdy controlled The mfluence of mstrumental parameters on the tkagmentaaon of clenbuterol was stu&ed Some results are presented that dlustrate the potential of electrospray lomzatlon for the mass spectrometrlc determmatlon of clenbuterol The senslttmty of the method IS discussed, and prelnnmary results on a blomedlcal apphcatron are presented Keywords Llquld chromatography, Mass spectrometly, &Agomsts, Clenbuterol, Pharmaceuticals, Unne Clenbuterol IS a /3-agonatlc drug used for the treatment of pulmonary diseases m humans and ammals Its chenucal structure IS closely related to that of catecholammes, and clenbuterol can interact vvlth adrenergc receptors In addltlon to their broncholytx propertles, /3-agonists possess strong repartltlonmg effects m meat-producmg ammals, owmg to reduced protem catabohsm and enhanced hpolysts, when admmltered at higher dosages The use of &agomsts as anunal feed addltwes has not yet been authored However, clenbuterol 1s the P-agomst most commonly and illegally used as a growth-promotmg agent for anunals Clenbuterol determmatrons have already been achieved either by unmunochemlcal methods [1,2] or physlco-chermcal methods Several procedures based on gas chromatography-mass spectrometry Correspondence to L Debrauwer, Laboratoue des Xinoblotlques, INRA,