Determination of calmodulin by competitive binding assay
β Scribed by Harry LeVine III; Naji Sahyoun; Pedro Cuatrecasas
- Book ID
- 102627728
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 490 KB
- Volume
- 152
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Radioligand binding assays are routinely utilized in laboratories throughout the world to study receptors and their related binding sites, carrier proteins, and enzymes. To accurately estimate equilibrium binding parameters, such as the equilibrium dissociation constant (Kd) and maximal number of bi
## Abstract A single step, separation free competitive binding reaction between the fluorescent antibiotic mithramycin and actinomycinβD for common binding sites on DNA coated 10 ΞΌm diameter microspheres is described. The fluorescence of the microspheres is measured with a flowcytometer. In the pre