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Competitive Binding Assay Using Fluorescence Resonance Energy Transfer for the Identification of Calmodulin Antagonists

✍ Scribed by Sharma, Bethel; Deo, Sapna K.; Bachas, Leonidas G.; Daunert, Sylvia


Book ID
127362093
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
281 KB
Volume
16
Category
Article
ISSN
1043-1802

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A Fluorescence Resonance Energy Transfer
✍ Dennis E. Epps; Mark A. Mitchell; Gary L. Petzold; John H. VanDrie; Roger A. Poo πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 110 KB

A sensitive fluorescence resonance energy transfer method was developed for the direct measurement of the dissociation constants of stromelysin inhibitors. The method is applied to the thiadiazole class of stromelysin inhibitors and it takes advantage of the fact that, upon binding to the active sit