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Flow cytometric competitive binding assay for determination of actinomycin-D concentrations

✍ Scribed by George C. Saunders; John C. Martin; James H. Jett; Ann Perkins

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 239 KB
Volume: 11
Category: Article
ISSN: 0196-4763
DOI: 10.1002/cyto.990110213

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A single step, separation free competitive binding reaction between the fluorescent antibiotic mithramycin and actinomycin‐D for common binding sites on DNA coated 10 μm diameter microspheres is described. The fluorescence of the microspheres is measured with a flowcytometer. In the presence of a constant amount of mithramycin, the microsphere fluorescence is inversely proportional to actinomycin‐D concentration.

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