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Determination of bongkrek acid and toxoflavin by high pressure liquid chromatography

โœ Scribed by A.G.J. Voragen; H.A.M. de Kok; A.J. Kelholt; H.A. Schols; Ko Swan Djien


Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
336 KB
Volume
9
Category
Article
ISSN
0308-8146

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A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated fr