Cauda epididymal spermatozoa of the golden hamster were labelled with the thiol-alkylating reagent, monobromobimane (MB). Female hamsters underwent uterine insemination with labelled spermatozoa at laparotomy under metofane anesthesia. All 12 females examined between 5 and 54 h postinsemination yiel
โฆ LIBER โฆ
Determination of acidity and nucleophilicity in thiols by reaction with monobromobimane and fluorescence detection
โ Scribed by Sardi, Florencia; Manta, Bruno; Portillo-Ledesma, Stephanie; Knoops, Bernard; Comini, Marcelo A.; Ferrer-Sueta, Gerardo
- Book ID
- 118241660
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 763 KB
- Volume
- 435
- Category
- Article
- ISSN
- 0003-2697
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A method for quantitative determination of biological thiols is presented. Thiols are converted to fluorescent derivatives by reaction with monobromobimane or monobromotrimethylammoniobimane. The derivatives are separated by ion-exchange chromatography and detected by fluorometry. Thiols that can be
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