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Normal development of hamster and rabbit eggs fertilized by spermatozoa labelled with the fluorescent thiol alkylating agent, monobromobimane

✍ Scribed by Fleming, Alan D. ;Cummins, James M. ;Kuehl, Thomas J. ;Seidel, George E. ;Yanagimachi, Ryuzo

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 657 KB
Volume: 237
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402370311

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✦ Synopsis

Cauda epididymal spermatozoa of the golden hamster were labelled with the thiol-alkylating reagent, monobromobimane (MB). Female hamsters underwent uterine insemination with labelled spermatozoa at laparotomy under metofane anesthesia. All 12 females examined between 5 and 54 h postinsemination yielded a total of 83/100 (83%) eggs in the process of fertilization or embryos. Under ultraviolet (W) exposure all exhibited a fluorescent tail which, in the 4-and &cell embryos, could be seen to be fraying or

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Labelling of living mammalian spermatozoa with the fluorescent thiol alkylating agent, monobromobimane (MB): Immobilization upon exposure to ultraviolet light and analysis of acrosomal status

✍ Cummins, James M. ;Fleming, Alan D. ;Crozet, Nicole ;Kuehl, Thomas J. ;Kosower, 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 759 KB

Living spermatozoa of seven mammalian species were treated with the thiol-alkylating fluorescent labelling compound, monobromobimane (MBBR). MB-labelling alone had no effect on sperm motility, nor on the time course or ability of golden hamster spermatozoa to undergo the acrosome reaction when capac