✦ LIBER ✦
Labelling of living mammalian spermatozoa with the fluorescent thiol alkylating agent, monobromobimane (MB): Immobilization upon exposure to ultraviolet light and analysis of acrosomal status
✍ Scribed by Cummins, James M. ;Fleming, Alan D. ;Crozet, Nicole ;Kuehl, Thomas J. ;Kosower, Nechama S. ;Yanagimachi, Ryuzo
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 759 KB
- Volume
- 237
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Living spermatozoa of seven mammalian species were treated with the thiol-alkylating fluorescent labelling compound, monobromobimane (MBBR). MB-labelling alone had no effect on sperm motility, nor on the time course or ability of golden hamster spermatozoa to undergo the acrosome reaction when capacitated in vitro. Exposure of MB-labelled spermatozoa to