Determination of 7-methylguanine, N2,N2-dimethylguanosine, and pseudouridine in ultrafiltrated serum of healthy adults by high-performance liquid chromatography

✍ Scribed by Heinrich Topp; Gernot Sander; Gesa Heller-Schöch; Gerhard Schöch

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 665 KB
• Volume: 161
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90650-6

✦ Synopsis

7-Methylguanine (m'Gua), N*,N*-dimethylguanosine (m:Guo), and pseudouridine ($) are degradation products from RNA turnover and can be used as markers for the whole-body turnover of mRNA-cap, tRNA, and rRNA (in healthy individuals, urinary excretion of these catabolites follows a regular pattern; the relative molar ratio of #:m'Gua:m$Guo is approximately 100: 196). HPLC methods were developed to measure serum concentrations of these RNA catabolites after deproteinization of the samples by ultrafiltration through microcollodion bags with a nominal exclusion M, of 12,400. For healthy adults the following values (mean f SD) were found: #, 2760 f 460 nmol/liter (n = 10); m'Gua, 129.7 5 24.0 nmol/liter (n = 13); m:Guo, 31.0 & 3.7 nmol/liter (n = 9). The relative molar ratio of these substances in serum derived from our data is approximately 100:4.7:1.1. 7-Methylguanosine (m'Guo) added to serum is to a large extent converted to the corresponding free base, m'Gua, the form which is excreted iTI urh2.