Determination of 5-aminolaevulinic acid dehydrase activity in erythrocytes by high-performance liquid chromatography
β Scribed by Helen Crowne; C.K. Lim; Diana Samson
- Book ID
- 108447736
- Publisher
- Elsevier Science
- Year
- 1981
- Weight
- 369 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0378-4347
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π SIMILAR VOLUMES
5-Aminolevulinic acid (ALA), the common precursor of all naturally occurring tetrapyrroles, forms a stable condensation product with 2-amino-3-hydroxynaphthafene which can be identified by its fluorescence. Separation of the compound by reversed-phase high-performance liquid chromatography on RPC-18
An isocratic reverse-phase \(\mathrm{C} 18 \mathrm{high}\)-performance liquid chromatography (HPLC) technique for methylmalonic acid (MMA) with fluorescence detection is described. MMA is extracted from an acidified sample (plasma, urine, etc.) with ethyl acetate. The extract is dried and derivatize