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Determinants of DNA Mismatch Recognition within the Polymerase Domain of the Klenow Fragment †

✍ Scribed by Thompson, Elizabeth H. Z.; Bailey, Michael F.; van der Schans, Edwin J. C.; Joyce, Catherine M.; Millar, David P.


Book ID
126481253
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
223 KB
Volume
41
Category
Article
ISSN
0006-2960

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A domain of the klenow fragment of Esche
✍ P. S. Freemont; Dr. D. L. Ollis; T. A. Steitz; C. M. Joyce 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 868 KB

The Klenow fragment of DNA polymerase I from Escherichia coli has two enzymatic activities: DNA polymerase and 3'-5' exonuclease. The crystal structure showed that the fragment is folded into two distinct domains. The smaller domain has a binding site for deoxynucleoside monophosphate and a divalent