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DNA polymerase I (Klenow fragment): Role of the structure and length of a template in enzyme recognition

✍ Scribed by T.I. Kolocheva; G.A. Nevinsky; V.A. Volchkova; A.S. Levina; V.V. Khomov; O.I. Lavrik


Book ID
115921674
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
306 KB
Volume
248
Category
Article
ISSN
0014-5793

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The Klenow fragment of DNA polymerase I from Escherichia coli has two enzymatic activities: DNA polymerase and 3'-5' exonuclease. The crystal structure showed that the fragment is folded into two distinct domains. The smaller domain has a binding site for deoxynucleoside monophosphate and a divalent