5'-Nucleotidase (5'-NT, E.C. 3.1.3.5) of cultured human and rodent cells was rendered soluble using the zwitterionic detergent Zwittergent 314. Optimal activity of 5'-NT was obtained when sonicated cells were incubated in solutions containing 0.75% (w/v) Zwittergent. A method was developed for the d
✦ LIBER ✦
Detergent solubilization of cardiac 5′-nucleotidase
✍ Scribed by G. Lutoslawaska; N. Jha; H. P. Baer
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 220 KB
- Volume
- 39
- Category
- Article
- ISSN
- 1420-682X
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