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Detection of topoisomerase inhibitor-induced DNA strand breaks and apoptosis by the alkaline comet assay

✍ Scribed by Thierry Godard; Edwige Deslandes; François Sichel; Jean-Michel Poul; Pascal Gauduchon

Book ID
117767001
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
766 KB
Volume
520
Category
Article
ISSN
1383-5718

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## Abstract Comet assay was applied to study induction and repair of DNA damage produced by bleomycin in barley genome. Experimental conditions were adapted to achieve efficient detection of both DNA single‐ and double‐strand breaks. Substantial increase of the parameter “% of DNA in tail” was obse