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Detection of SV40 T antigen with labelled antibodies: Radioimmunoassay and autoradiography

✍ Scribed by R. M. Flügel; T. Crefeld; K. Munk

Publisher: John Wiley and Sons
Year: 1977
Tongue: French
Weight: 806 KB
Volume: 19
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910190509

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✦ Synopsis

Abstract

SV40 T antigen, partially purified from SV80, a human cell line, has been used to develop a sensitive solid phase radioimmunoassay. In this assay SV40 T antigen was immobilized on microtiter plates, reacted with hamster anti‐SV40 T antibody and subsequently with ^14^C‐labelled anti‐hamster IgG. The test detected approximately 20 ng of partially purified SV40 T antigen and is rapid and more convenient than the complement fixing reaction. An immunoautoradiographic method which permits the determination of the cellular localization of SV40 T antigen and which is possibly more sensitive than the indirect immunofluorescence method is also described.

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