Detection of Southern African human immunodeficiency virus type 1 subtypes by polymerase chain reaction: evaluation of different primer pairs and conditions
β Scribed by Susan Engelbrecht; Estrelita J. van Rensburg
- Book ID
- 113256561
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 723 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0166-0934
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## Abstract A highly sensitive twoβstep polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIVβ1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an interna
An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR