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Detection of single-strand breaks and formamidopyrimidine-DNA glycosylase-sensitive sites in DNA of cultured human fibroblasts

✍ Scribed by Stefan Czene; Mats Harms-Ringdahl

Book ID
116157962
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
741 KB
Volume
336
Category
Article
ISSN
0921-8777

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In vitro exposure of normal human diploid fibroblasts (strain VH-10) to ethylene oxide (EtO) induced DNA strand breaks in the dose range of 2.5-30 mMh of EtO. Alkaline DNA unwinding (ADU), neutral filter elution (NFE), pulsed field gel electrophoresis (PFGE), and the comet assay were used to measure