## Abstract Capillary electrochromatography (CEC) was employed as a rapid and highβefficiency method for the isocratic separation of all 20 important phenylthiohydantoin (PTH) amino acids, the end products of Edman degradation during __N__βterminal protein sequencing. For this purpose, 75Β ΞΌm ID fus
Detection of phenylthiohydantoin derivatives of amino acids by SIMS
β Scribed by A. Benninghoven; V. Anders
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 209 KB
- Volume
- 19
- Category
- Article
- ISSN
- 1076-5174
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π SIMILAR VOLUMES
Phenylthiohydantoin (PTH) derivatives of all 20 common amino acids can be separated by high-pressure liquid chromatography. By using a Waters reversed-phase C18 column eluted with a concave ethanol gradient in ammonium acetate, pH 5.1, all PTH derivatives were eluted in less than 30 min. The NH,-ter
The N-terminal phenylthiocarbamoyl (PTC) derivatives of peptides and the phenylthiohydantoin (PTH) derivatives of amino acids are the two major types of products generated in the Edman protein sequencing method. Understanding the fragmentation pathways of these species should facilitate structural e