Detection of N-acetylcysteine, cysteine and their disulfides in urine by liquid chromatography with a dual-electrode amperometric detector
β Scribed by Julie A. Stenken; Deanna L. Puckett; Susan M. Lunte; Craig E. Lunte
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 487 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0731-7085
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β¦ Synopsis
A method for the determination of N-acetylcysteine, cysteine and their disulfides in urine is described. The thiols and disulfides are separated by reversed-phase ion-pair chromatography with octyl sodium sulfate as the ion-pairing reagent and detected with a dual-electrode amperometric detector using Au/Hg amalgam electrodes. Both the thiols and disulfides are detected with this system. In addition, dimers and mixed disulfides can be detected individually.
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