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Detection of N-acetylcysteine, cysteine and their disulfides in urine by liquid chromatography with a dual-electrode amperometric detector

✍ Scribed by Julie A. Stenken; Deanna L. Puckett; Susan M. Lunte; Craig E. Lunte


Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
487 KB
Volume
8
Category
Article
ISSN
0731-7085

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✦ Synopsis


A method for the determination of N-acetylcysteine, cysteine and their disulfides in urine is described. The thiols and disulfides are separated by reversed-phase ion-pair chromatography with octyl sodium sulfate as the ion-pairing reagent and detected with a dual-electrode amperometric detector using Au/Hg amalgam electrodes. Both the thiols and disulfides are detected with this system. In addition, dimers and mixed disulfides can be detected individually.


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