Determination of N-nitrosopiperidine in beers by liquid chromatography with reductive amperometric detection at a hanging mercury drop electrode

✍ Scribed by M.Aranzazu Goicolea; Zuriñe Gomez de Balugera; M.Jesús Portela; Ramón J. Barrio

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 676 KB
• Volume: 305
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)00443-p

✦ Synopsis

Liquid chromatographic separation prior to reductive electrochemical determination at the hanging mercury drop electrode is used to determine N-nitrosopiperidine (NPIP). A mobile phase of methanol-acetonitrile-phosphate buffer, pH 3.5 (57:38:5) is used with the detector operated in the direct current (DC) mode at Ep = -1250 mV. Techniques for oxygen removal and electrochemical considerations are presented. The detection limit is 0.2 ng injected with a relative standard deviation (n = 10) of 2.8%. The method can be applied to the determination of NPIP in beers. Conditions for the prior extraction of NPIP from beer samples have been established using solid phase extraction cartridges.