In Vivo Monitoring of the Thiols in Rat Striatum by Liquid Chromatography with Amperometric Detection at a Functionalized Multi-Wall Carbon Nanotubes Modified Electrode

✍ Scribed by Xu-Ni Cao; Li Lin; Yue-Zhong Xian; Wen Zhang; Yun-Feng Xie; Li-Tong Jin

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 112 KB
• Volume: 15
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200390111

✦ Synopsis

Abstract

A new chemically modified electrode (CME) was fabricated, which was based on the immobilization of multi‐wall carbon nanotubes fuctionalized with carboxylic group (MWNT‐COOH). The results indicated that the CME exhibited efficiently electrocatalytic oxidation for L‐cysteine and glutathione with relatively high sensitivity, stability and long‐life. Coupled with HPLC, the MWNT‐COOH CME was utilized for amperometric detection of the thiols. The peak currents of L‐cysteine and glutathione were linear to their concentrations ranging from 3.0×10^−7^ to 1.0×10^−3^ mol/L with the calculated detection limit (S/N=3) of 1.2×10^−7^, 2.2×10^−7^ mol/L, respectively. The method had been successfully applied to assess the contents of L‐cysteine and glutathione in rat striatal microdialysates.