Detection of Mutant K-RAS Sequences in the Urine of Cancer Patients
β Scribed by O.I. Serdyuk; I.V. Botezatu; V.P. Shelepov; G.I. Potapova; R.P. Alekhina; Yu.K. Molyaka; V.S. Anan'ev; V.I. Knysh; O.S. Melkonyan; S.R. Umanskii; A.V. Lichtenshtein
- Book ID
- 110365478
- Publisher
- Springer US
- Year
- 2001
- Tongue
- English
- Weight
- 425 KB
- Volume
- 131
- Category
- Article
- ISSN
- 0007-4888
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Mutant-enriched PCR was applied to the detection of mutations at codons I 2 and I 3 of K-ras genes in the stools of patients with colorectal cancer. Mutations were analyzed in stool samples obtained prior to surgery. Resected tumor specimens were screened for K-ras mutations by PCR-mediated RFLP ana
From 10 to 30% of lung carcinomas examined to date contain mutant K-ras genes. We report here that the mutant-allele-specific amplification (MASA) method may be useful for detection of the K-ras mutations in cells obtained from the sputum of patients with lung cancer. The PCR product from one of fiv