A simple procedure for reusing polyvinylidene difluoride membranes with bound proteins reacted with antibodies and developed by the nitro blue tetrazolium method (M. S. Blake, K. H. Johnston, G. J. Russell-Jones, and E. C. Gotschlich (1984) Anal. Biochem. 136, 175-179) is described. The blue diforma
Detection of lipopolysaccharides blotted to polyvinylidene difluoride membranes
β Scribed by Murry A. Stein; Sylvia A. McAllister; Kenneth H. Johnston; Dana L. Diedrich
- Book ID
- 107714918
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 597 KB
- Volume
- 188
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A simple method of blotting glycosphingolipids from a high-performance thin-layer chromatography (HPTLC) plate to a polyvinylidene difluoride (PVDF) membrane is described. The developed HPTLC plate is dipped in a solvent mixture (isopropanol/0.2\% \(\mathbf{C a C l}_{2}\) / methanol, \(40 / 20 / 7\)
A simple, rapid method for the analysis of glycosphingolipid that combines "thin-layer chromatography (TLC) blotting" and mass spectrometry is reported. Glycosphingolipids developed by TLC were transferred to a polyvinylidene difluoride membrane by TLC blotting, after which the glycosphingolipid ban