Improving the detection of proteins after transfer to polyvinylidene difluoride membranes
✍ Scribed by Jean-Charles Sanchez; Florence Ravier; Christian Pasquali; Séverine Frutiger; Nicole Paquet; Bengt Bjellqvist; Denis F. Hochstrasser; Graham J. Hughes
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 433 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0173-0835
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📜 SIMILAR VOLUMES
A rapid and simple method for the quantitation of stained proteins bound to polyvinylidene difluoride (PVDF) membranes via the elution of Coomassie brilliant blue R-250 is described. A mixture of standard proteins was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrob
A simple, rapid method for the analysis of glycosphingolipid that combines "thin-layer chromatography (TLC) blotting" and mass spectrometry is reported. Glycosphingolipids developed by TLC were transferred to a polyvinylidene difluoride membrane by TLC blotting, after which the glycosphingolipid ban
S-Carboxymethylation of proteins transferred onto polyvinylidene difluoride membranes followed by in situ protease digestion and amino acid microsequencing S-Carboxymethylation of immobilized proteins, separated by gel electrophoresis and transblotted to polyvinylidene difluoride (PVDF) membranes is