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Direct Mass Spectrometric Analysis of Glycosphingolipid Transferred to a Polyvinylidene Difluoride Membrane by Thin-Layer Chromatography Blotting

✍ Scribed by T. Taki; D. Ishikawa; S. Handa; T. Kasama

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
267 KB
Volume
225
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple, rapid method for the analysis of glycosphingolipid that combines "thin-layer chromatography (TLC) blotting" and mass spectrometry is reported. Glycosphingolipids developed by TLC were transferred to a polyvinylidene difluoride membrane by TLC blotting, after which the glycosphingolipid band on the membrane was excised and placed on a mass spectrometer probe tip, and a few microliters of triethanolamine was added as the matrix. The sample was analyzed by secondary ion mass spectrometry. About (1 \mu \mathrm{g}) of glycosphingolipid subjected to TLC can be analyzed. The major advantage of this method is that glycosphingolipid can be analyzed structurally without purification by repeated column chromatography. ic 1995 Academic Press, Inc.

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Direct matrix-assisted laser desorption/
Direct matrix-assisted laser desorption/ionization mass spectrometric analysis of glycosphingolipids on thin layer chromatographic plates and transfer membranes
✍ JoΓ«lle Guittard; Xiaoping L. Hronowski; Catherine E. Costello πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 455 KB

Results are reported for analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of native glycosphingolipids (GSLs) after development on thin layer chromatographic plates and after heat transfer of the GSLs from the plates to several types of polymer m