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Detection of Lipase Activity on Ultrathin-Layer Isoelectric Focusing Gels

✍ Scribed by O.M. Nuero; R. Garcialepe; C. Lahoz; F. Santamaria; F. Reyes

Book ID
102560003
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
348 KB
Volume
222
Category
Article
ISSN
0003-2697

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Polyacrylamide gels were stained with the sialidase substrate 2'-(4-methylumbelhferyl)-a-D-N-acetylneuraminic acid showing the activity of Vibrio cholerae and Clostridium sordellii sialidases in the gels after electrophoresis. With this fluorogenic method minimum sialidase activities of 5 r.JJ could