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Detection of human cytomegalovirus DNA in immunocompromised children by polymerase chain reaction

✍ Scribed by Nishihara, Hidehiro; Ito, Masahiro; Matsumoto, Naoko; Nakano, Takashi; Ihara, Toshiaki; Kamiya, Hitoshi; Sakurai, Minoru


Book ID
122503349
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
644 KB
Volume
3
Category
Article
ISSN
0928-0197

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Human cytomegalovirus infection during c
✍ Motohiro Shibata; Tsuneo Morishima; Makoto Terashima; Hiroshi Kimura; Kiyotaka K πŸ“‚ Article πŸ“… 1990 πŸ› Springer-Verlag 🌐 English βš– 858 KB

Polymerase chain reaction (PCR) technique was applied to detect cytomegalovirus (CMV) DNA. Two pairs of synthetic oligonucleotide primers were used to amplify DNA from the immediate early 1 and the late antigen genes of CMV, respectively. Either primer sets could detect as few as 0.01 plaque-forming