𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridization

✍ Scribed by Dr.; Dr. S. Myint; S. Siddell; D. Tyrrell

Book ID
102378901
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
480 KB
Volume
29
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridisation. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single-stranded RNA probe. Nucleic acid hybridisation is shown to be a sensitive technique for the diagnosis of HCV 229E infections.

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