𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of hepatitis C virus (HCV) RNA in paraffin embedded tissue sections of human liver of non-A, non-B hepatitis patients by in situ hybridization

✍ Scribed by Dr. Shuhei Yamada; Takehiko Koji; Masayuki Nozawa; Kendo Kiyosawa; Paul K. Nakane

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
668 KB
Volume
6
Category
Article
ISSN
0887-8013

No coin nor oath required. For personal study only.

✦ Synopsis

Localization of hepatitis C virus (HCV) RNA was investigated by non-radioactive in situ hybridization in human liver specimens of chronic non-A, non-B (NANB) hepatitis patients who were seropositive for antibodies to HCV (anti-HCV). For in situ hybridization, T-T dimerized synthetic oligodeoxynucleotide probes were used and DNAs hybridized in situ were detected immunohistochemically using specific antibodies against T-T dimer. The data demonstrates that HCV-RNA was localized in the cytoplasm of hepatocytes in human liver biopsies obtained from the patients with chronic NANB hepatitis seropositive for anti-HCV.

πŸ“œ SIMILAR VOLUMES

Detection of hepatitis c virus rna genom
Detection of hepatitis c virus rna genome in liver tissue by nonisotopic in situ hybridization
✍ MD Johnson Y. N. Lau; Gary L. Davis πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 464 KB

## Abstract A rapid technique for the detection of hepatitis C virus (HCV) RNA in liver section using noniso‐topic in situ hybridization was described. Only 3 of the 10 patients seropositive for antibody to HCV and HCV RNA had detectable HCV RNA in the hepatocytes (1%, 2%, and 15% of cells positive

Detection of hepatitis B virus DNA in pa
Detection of hepatitis B virus DNA in paraffin-embedded liver tissues in chronic hepatitis B or non-A, non-B hepatitis using the polymerase chain reaction
✍ Michiko Shindo; Tadao Okuno; Ken Arai; Masayuki Matsumoto; Makoto Takeda; Kei Ka πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 553 KB

We developed a polymerase chain reaction assay for the direct detection of hepatitis B virus in paraffin-embedded liver tissue and applied this assay to determine whether hepatitis B virus DNA exists in livers with chronic hepatitis non-A, non-B. Fifty five liver biopsy samples were studied: 11 from

Non-A, non-B chronic hepatitis is chroni
Non-A, non-B chronic hepatitis is chronic hepatitis C: A sensitive assay for detection of hepatitis C virus RNA in the liver
✍ Kazuhiko Hosoda; Masao Omata; Osamu Yokosuka; Naoya Kato; Masao Ohto πŸ“‚ Article πŸ“… 1992 πŸ› John Wiley and Sons 🌐 English βš– 500 KB

To study the role of hepatitis C virus in non-A, non-B chronic hepatitis, 49 liver biopsy samples from 40 patients with non-A, non-B chronic hepatitis and 9 control patients were analyzed by complementary DNA/polymerase chain reaction. Two segments of the HCV genome, one in the nonstructural region

Detection of hepatitis C virus genome in
Detection of hepatitis C virus genome in formalin-fixed paraffin-embedded liver tissue by in situ reverse transcription polymerase chain reaction
✍ George K. K. Lau; Jane W. S. Fang; P. C. Wu; Gary L. Davis; Johnson Y. N. Lau πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 470 KB

## Abstract Detection of hepatic hepatitis C virus RNA and antigens is difficult since their expression is very low. The technique of in situ reverse transcription polymerase chain reaction (IS‐RT‐PCR) was developed for the detection and localization of HCV RNA in formalin‐fixed paraffin‐embedded l